Using Multiplex Testing to Unravel Responses to Acute and Chronic Viral Infections, New Webinar Hosted by Xtalks
TORONTO, November 14, 2018 (Newswire.com) - Join Philip J. Norris, M.D., Vice President of Research and Scientific Programs at Vitalant, for a live webinar on Wednesday, Dec. 5, 2018, at 12 p.m. EST.
The immune response to viral infections is essential for efficient clearance of acute infection, yet in chronic viral infections, this response can lead to inflammation and immunopathology. Understanding the global immune response requires measurement of multiple parameters simultaneously since the arms of the immune system are interconnected and do not operate in isolation. One of the limitations in assessing immune responses is availability of immune cells, which require specialized collection and cryopreservation, and access to sufficient volume of plasma or serum to assess multiple soluble analytes. Cytokines and chemokines make attractive targets for immune profiling given their role in intercellular signaling and their presence in the peripheral circulation. One solution that has been widely adopted in the last decade is multiplex testing, which allows a much more comprehensive assessment of protein expression than previously achievable.
The most valuable samples to test in understanding human disease are clinical samples obtained from study subjects with acute or chronic infections. However, one of the key limiting factors in testing animal or human clinical samples is that cytokine levels tend to be near the limit of detection in serum or plasma. This webinar will discuss how to build multiplex panels to maximize efficiency in testing human or animal samples in terms of cost and sample volume limitations. After construction of a candidate panel, validation is necessary and we will review a method to validate assay performance before testing valuable clinical specimens. Other potential limitations of clinical sample testing are the possibility of long-term storage or multiple freeze-thaw cycles of samples prior to testing, and the effect of these variables will be discussed. During testing, QC is necessary to control for assay performance and to monitor for batch testing effects, plate-to-plate variation and lot-to-lot variation. Methods to monitor and control for these factors will be discussed.
Once testing is operational and validated, the power of multiplex testing allows insight into a variety of disease states. This presentation will focus on cytokine network evolution in acute viral infections, as well as how chronic inflammation associates with immune pathology in chronic viral infections. These data will serve as a template for how multiplex testing can be applied to the study of a variety of animal models and human diseases using ex vivo samples.
For more information or to register for this event, visit: Using Multiplex Testing to Unravel Responses to Acute and Chronic Viral Infections.
ABOUT XTALKS
Xtalks, powered by Honeycomb Worldwide Inc., is a leading provider of educational webinars to the global life science, food and medical device community. Every year thousands of industry practitioners (from life science, food and medical device companies, private & academic research institutions, healthcare centers, etc.) turn to Xtalks for access to quality content. Xtalks helps Life Science professionals stay current with industry developments, trends and regulations. Xtalks webinars also provide perspectives on key issues from top industry thought leaders and service providers.
To learn more about Xtalks, visit http://xtalks.com.
For information about hosting a webinar, visit http://xtalks.com/why-host-a-webinar/.
Contact:
Nima Rajan
Tel: +1 (416) 977-6555 ext 352
Email: nrajan@xtalks.com
Source: Xtalks
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