New Feline Insulin ELISA Kit Released by Creative Diagnostics
Creative Diagnostics introduces Feline Insulin ELISA kits for measurement of insulin levels in feline serum or plasma samples.
New York, New York, March 1, 2016 (Newswire.com) - As a leading manufacturer of ELISAs, Creative Diagnostics continues to grow its immunoassay portfolio for metabolic research with the introduction of the Feline Insulin ELISA kits, providing a method for measurement of insulin levels in feline serum or plasma samples.
Insulin is a peptide hormone produced by the pancreas, which is central to regulating carbohydrate and fat metabolism in the body. It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues. Insulin stops the use of fat as an energy source by inhibiting the release of glucagon.
With the exception of the metabolic disorder diabetes mellitus and metabolic syndrome, insulin is provided within the body in a constant proportion to remove excess glucose from the blood, which otherwise would be toxic. When blood glucose levels fall below a certain level, the body begins to use stored sugar as an energy source through glycogenolysis, which breaks down the glycogen stored in the liver and muscles into glucose, which can then be utilized as an energy source. As a central metabolic control mechanism, its status is also used as a control signal to other body systems (such as amino acid uptake by body cells). In addition, it has several other anabolic effects throughout the body.
The Feline Insulin ELISA is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the insulin molecule. During incubation, insulin in the sample reacts with peroxidase-conjugated anti-insulin antibodies and anti-insulin antibodies bound to the microtitration well.
After a simple washing step that removes unbound enzyme labelled antibody, the bound conjugate is detected by reaction with 3, 3’-5, 5’-tetramethylbenzidine (TMB). The reaction is stopped by the addition of acid, giving a colorimetric endpoint that can be read spectrophotometrically.
Like many other Creative Diagnostics ELISA kits, these kits are complete, flexible and thoroughly validated. Each kit is taken through rigorous fit-for-purpose validation and stability testing to ensure accuracy, sensitivity, and specificity.
For more information or to order the Feline Insulin ELISA kits or other products, please feel free to call us at 1-631-624-4882 or email us to info@creative-diagnostics.com.
About Creative Diagnostics
Creative Diagnostics is a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components and critical assay reagents. We provide contract biologic R&D and manufacturing services to the diagnostic manufacturers along with GMP biologics manufacturing for the biopharmaceutical market. Our goal is to provide a trusted source for all your assay development and manufacturing needs.
We also assist clients in rapidly developing, manufacturing and commercializing point of care lateral flow, flow through and ELISA assays for any market segment. We develop using fluorescent, visual colloidal gold and paramagnetic labels, and high sensitivity, quantitative and reader-based tests are our specialty.
Contact
Creative Diagnostics
Phone: 1-631-624-4882
Fax: 1-631-938-8221
Address: 45-16 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-diagnostics.com