Hydrogels and Technologies for Successful 3D Cell Culture and Organoid Culture Focused on Cellular Assay Development and Drug Screening, a New Webinar Hosted by Xtalks
3D culture and organoids are fast-becoming key in vitro models for studying tissues, disease and drugs. Stem Pharm used their synthetic hydrogel platform to create the vascular tubulogenesis and developmental neurotoxicity assays discussed in this webinar.
TORONTO, May 24, 2018 (Newswire.com) - This webinar will provide background on the rationale for use of synthetic hydrogels for advanced cellular applications in the drug discovery arena and will highlight techniques and technologies used to analyze outputs of these applications.
Stem Pharm Inc. has developed a synthetic hydrogel platform that allows the design and optimization of substrates for cell expansion, differentiation and screening applications including 3D cell culture and organoid models. Through control of the substrate mechanical properties and adhesion ligand presentation, and utilizing chemistries that maintain cellular health and function, Stem Pharm provides cell-specific biomaterials for advanced cellular assay platforms and specialized cell expansion and differentiation applications.
For many applications, these hydrogels provide advantages over animal-derived biomaterials such as the Engelbreth-Holm-Swarm mouse sarcoma-derived products marketed as Matrigel®, Geltrex® and Cultrex®. As one example, Stem Pharm has developed a vascular tubulogenesis hydrogel that enables high throughput screening (HTS) for vascular disruptors utilizing human umbilical vein endothelial cells (HUVEC) or iPSC-derived endothelial cells.
Use of this hydrogel provides advantages over assay platforms that use Matrigel®, and similar products, that are challenging in an HTS workflow due to their temperature sensitivity and lot-to-lot variability. The Stem Pharm hydrogel platform is flexible for use in standard cell culture workflows not requiring complex bioprinting methodologies and is suitable for co-culture and 3D organoid applications. Using Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader, organoids can be formed and maintained in multi-well plates while adhering to the hydrogel rather than growing en masse in suspension cultures. This facilitates their use for toxicity or efficacy screening applications, including those requiring imaging readouts. In another example, a neural organoid model enabled by these hydrogels has been developed which produced multicomponent neural constructs with 3D neuronal and glial organization, organized vascular networks and microglia with ramified morphologies (Schwartz et al (2015), PNAS 112, 12516-12521 and Barry et al. (2017), Exp Biol Med 242, 1679-1689). This model was utilized in a developmental neurotoxicity screen and demonstrated to be very reproducible both well-to-well and between independent experiments.
Join the live session on Tuesday, June 5, 2018, at 11 a.m. EDT (4 p.m. BST/UK), with guest speaker Connie Lebakken, Ph.D., president and chief operating officer at Stem Pharm Incorporated. The discussion, including a Q&A with the audience, will benefit scientists, engineers and professionals in life science fields who are interested in biomaterials, hydrogels, 3D cell culture, organoids or drug discovery.
For more information about this complimentary webinar, visit Versatile Synthetic Substrates for Cellular Assay Development and 3D Organoid Culture and Screening.
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Contact:
Nima Rajan
Tel: +1 (416) 977-6555 ext 352
Email: nrajan@xtalks.com
Source: Xtalks
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